GenePage for the btsS gene of Escherichia coli K-12

Primary Gene Name: btsS
EcoGene Accession Number: EG12007
K-12 Gene Accession Number: ECK2118
MG1655 Gene Identifier: b2126
Gene Name Mnemonic: Brenztraubensäure
Alternate Gene Symbols: yehU
Description: Stationary phase sensor kinase regulating btsT; pyruvate-responsive
  # bp Upstream # bp Downstream
MW: 62092.46 ---------561 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2212959
Left Intergenic Region

Name: btsR_btsS

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 2212959

Right_end: 2212962

Centisome: 47.68

Genomic Address
Minute or Centisome (%) = 47.68
Right End: 2214644
Right Intergenic Region

Name: btsS_mlrA

Length: 221 bp gap

Orientation: Divergent

Left_end: 2214645

Right_end: 2214865

Centisome: 47.71

BtsS binds extracellular pyruvate, which can be produced during serine catabolism, with high affinity and thus senses the onset of nutrient limitation in amino acid rich media, signaling BtsR to induce transcription of the pyruvate transporter gene btsT (Behr, 2017). BtsR(YehT) is the cognate response regulator for BtsS(YehU). The btsT(yjiY) gene is transcriptionally regulated by the BtsSR(YehUT) two-component system; the response regulator BtsR(YehT) binds the btsT(yjiY) promoter and activates btsT(yjiY) transcription; btsT(yjiY) is strongly induced in stationary phase when cells are grown on amino acids or peptides; yhjX is strongly repesssed by BtsSR(YehUT) but it appears be an indirect target since tight binding of BtsS(YehU) to the yhjX promoter was not detected; BtsSR(YehUT) is suggested to be part of a stationary phase control network (Kraxenberger, 2012). The YpdAB-regulated transporter gene yhjX is induced by high (>600 micromolar) concentrations of pyruvate while the BtsSR-regulated high-affinity pyruvate symporter gene btsT is induced by low (<5 micromolar) concentrations of pyruvate (Behr, 2017b). The YpdAB and BtsSR(YehUT) two-component regulators are paralogous; these two systems may be interconnected since they both regulate btsT(yjiY), directly by BtsSR(YehUT) and indirectly by YpdAB; although not noted by the authors, both sytems also regulate yhjX, indirectly by BtsSR(YehUT) and directly by YpdAB; the transporter genes yhjX and btsT(yjiY) are the only two genes significantly regulated by YpdAB and BtsSR(YehUT) (Fried, 2013). The mnemonic for the bts genes is BrenzTraubenSäure, the name given by Jöns Jakob Berzelius to pyruvic acid when he first synthesized pyruvic acid in 1835 (Behr, 2017). A btsSR ypdAB quadruple mutant has a higher level of persistor cells than wildtype after ampicillin treatment of stationary phase, but not exponential phase, cells (Vilhena, 2017).

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