GenePage for the iceT gene of Escherichia coli K-12

Primary Gene Name: iceT
EcoGene Accession Number: EG12136
K-12 Gene Accession Number: ECK2073
MG1655 Gene Identifier: b2077
Gene Name Mnemonic: Iron citrate efflux
Alternate Gene Symbols: mdtD; yegB
Description: Putative citrate/iron-citrate/zinc-citrate efflux transporter; putative arabinose exporter
  # bp Upstream # bp Downstream
MW: 50900.89 ---------471 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2161464
Left Intergenic Region

Name: mdtC_iceT

Length: 0 bp

Orientation: Codirectional+

Left_end: Null

Right_end: Null

Centisome: 46.57

Genomic Address
Minute or Centisome (%) = 46.57
Right End: 2162879
Right Intergenic Region

Name: iceT_baeS

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 2162876

Right_end: 2162879

Centisome: 46.60

Salmonella IceT(MdtD) has been characterized as a citrate and iron-citrate efflux transporter (Frawley, 2013). iceT(mdtD) mutants grow slower in the presence of zinc at high cell density and have a dramatically increased intracellular free zinc concentration after zinc shock, indicating that IceT may export zinc-citrate; the authors note that "it is likely that zinc is co-transported in a complex with other ligands that are substrates of MdtD" (Wang, 2013). Plasmid-mediated mdtABC-iceT(mdtD) overexpression confers resistance to deoxycholate and novobiocin in an acrAB mutant background but the same resistances are observed with mdtABC overexpression, indicating that iceT(mdtD) does not confer multidrug resistance (Nishino, 2001; Nagakubo, 2002). Chromosomal mdtABC-iceT(mdtD) expression induced by plasmid-mediated BaeR overproduction increases resistance to deoxycholate and novobiocin in an acrAB mutant; mutations in mdtB and mdtC but not iceT(mdtD) abolish this BaeR-induced resistance phenotype (Baranova, 2002). Consistent with arabinose export, an iceT(mdtD) mutant has increased intracellular arabinose, but not xylose; other compounds were not tested (Koita, 2012).

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