GenePage for the bcsB gene of Escherichia coli K-12

Primary Gene Name: bcsB
EcoGene Accession Number: EG12259
K-12 Gene Accession Number: ECK3517
MG1655 Gene Identifier: b3532
Gene Name Mnemonic: Bacterial cellulose synthesis
Alternate Gene Symbols: yhjN
Description: Cellulose synthase, regulatory subunit; binds cyclic-di-GMP; periplasmic, membrane-anchored
  # bp Upstream # bp Downstream
MW: 86023.67 ---------779 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3690268
Left Intergenic Region

Name: bcsZ_bcsB

Length: 6 bp gap

Orientation: Codirectional-

Left_end: 3690262

Right_end: 3690267

Centisome: 79.50

Genomic Address
Counterclockwise
Minute or Centisome (%) = 79.50
Right End: 3692607
Right Intergenic Region

Name: bcsB_bcsA

Length: 10 bp gap

Orientation: Codirectional-

Left_end: 3692608

Right_end: 3692617

Centisome: 79.55

Inverted membrane vesicles prepared from E. coli cells overexpressing E. coli K-12 BcsA and BcsB can synthesize cellulose in the presence of c-di-GMP (Omadjela, 2013). Overproduction of BcsB enhances exopolysaccharide production, flocculation (cell aggregation) in liquid culture, and biofilm formation, possibly incuding cellulose production, which most K-12 strains cannot make because of a mutation in bcsQ (Nguyen, 2014). The bcsQ' domestication in-frame stop codon pseudogene defect in K-12 strain W3110 has been repaired, restoring cellulose production, colony morphology, biofilm architecture, and the divergent bcs operons' functions to a presumably wildtype status (Serra, 2013). The presence of an in-frame stop codon mutation in the bcsQ' gene abolishes cellulose production and bcs divergent operon function in E. coli K-12. BcsB has a predicted 25 aa type I signal peptide.

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BamHI EcoRI HindIII