GenePage for the coaE gene of Escherichia coli K-12

Primary Gene Name: coaE
EcoGene Accession Number: EG12312
K-12 Gene Accession Number: ECK0103
MG1655 Gene Identifier: b0103
Gene Name Mnemonic: Coenzyme A
Alternate Gene Symbols: yacE
Description: Dephospho-CoA kinase; final step in CoA synthesis
  # bp Upstream # bp Downstream
MW: 22621.71 ---------206 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 112599
Left Intergenic Region

Name: zapD_coaE

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 112599

Right_end: 112599

Centisome: 2.43

Genomic Address
Counterclockwise
Minute or Centisome (%) = 2.43
Right End: 113219
Right Intergenic Region

Name: coaE_guaC

Length: 224 bp gap

Orientation: Divergent

Left_end: 113220

Right_end: 113443

Centisome: 2.44

The coaE mutants in the Keio collection have been shown to harbor an intact copy of coaE in addition to the deletion allele, indicating that coaE could not be deleted and that it is an essential gene (Yamamoto, 2009). The coaE is essential for growth as tested by genetic footprinting (Hare, 2001). The N-terminus of a recombinant CoaE protein was sequenced but this is not verification for the start codon of fMet cleavage since plasmid gene expression was forced at the ATG and an Ala was added during cloning between the fMet and the second Arg residue (Mishra, 2001).

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BamHI EcoRI HindIII