GenePage for the btsT gene of Escherichia coli K-12

Primary Gene Name: btsT
EcoGene Accession Number: EG12586
K-12 Gene Accession Number: ECK4344
MG1655 Gene Identifier: b4354
Gene Name Mnemonic: Brenztraubensäure
Alternate Gene Symbols: oppE?; yjiY
Description: Pyruvate/H+ symporter; BtsSR-regulated in stationary phase
  # bp Upstream # bp Downstream
MW: 77323.88 ---------716 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4589129
Left Intergenic Region

Name: yjiX_btsT

Length: 49 bp gap

Orientation: Codirectional-

Left_end: 4589080

Right_end: 4589128

Centisome: 98.87

Genomic Address
Minute or Centisome (%) = 98.87
Right End: 4591279
Right Intergenic Region

Name: btsT_tsr

Length: 377 bp gap

Orientation: Divergent

Left_end: 4591280

Right_end: 4591656

Centisome: 98.91

BtsT is a high-affinity pyruvate/H+ symporter regulated by BtsSR (Kristoficova, 2017). btsT(yjiY) is transcriptionally regulated by the BtsSR(YehUT) two-component system; btsT(yjiY) expression is absent in a btsSR(yehUT) mutant; the response regulator BtsR(YehT) binds the btsT(yjiY) promoter as its only DNA target; btsT(yjiY) is induced in stationary phase when cells are growing on amino acids or peptides (Kraxenberger, 2012). The YpdAB-regulated transporter gene yhjX is induced by high (>600 micromolar) concentrations of pyruvate while the BtsSR-regulated high-affinity pyruvate symporter gene btsT is induced by low (<5 micromolar) concentrations of pyruvate (Behr, 2017b). The YpdAB and BtsSR(YehUT) two-component regulators are paralogous; these two systems may be interconnected since they both regulate btsT(yjiY), directly by BtsSR(YehUT) and indirectly by YpdAB; although not noted by the authors, both sytems also regulate yhjX, indirectly by BtsSR(YehUT) and directly by YpdAB; the transporter genes yhjX and btsT(yjiY) are the only two genes significantly regulated by YpdAB and BtsSR(YehUT) (Fried, 2013). A suggestion that yjiY is oppE is based upon genetic mapping that places oppE in this region and the peptide utilization phenotype of its paralog cstA. In Salmonella typhimurium, a btsT(yjiY) mutant affects the formation of flagella, has reduced colonization of mouse intestine, and is defective in the uptake of some peptides (Garai, 2015). A btsSR ypdAB quadruple mutant has a higher level of persistor cells than wildtype after ampicillin treatment of stationary phase, but not exponential phase, cells (Vilhena, 2017). Paralogs cstA and yjiY are both in operons with paralogous small proteins of unknown function, ybdD and yjiX, respectively. HT_Cmplx48_Mem: SecA+YdgA+YjiY.

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