GenePage for the flu gene of Escherichia coli K-12

Primary Gene Name: flu
EcoGene Accession Number: EG12686
K-12 Gene Accession Number: ECK1993
MG1655 Gene Identifier: b2000
Gene Name Mnemonic: Fluffing
Alternate Gene Symbols: agn; agn43; yeeQ; yzzX
Description: Antigen 43, phase-variable bipartite outer membrane protein; Ag43; fluffing protein; CP4-44 defective prophage; affects surface properties, piliation, colony morphology
  # bp Upstream # bp Downstream
Verified Start MW: 106825.44 ---------1039 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2071539
Left Intergenic Region

Name: isrC_flu

Length: 27 bp gap

Orientation: Codirectional+

Left_end: 2071512

Right_end: 2071538

Centisome: 44.63

Genomic Address
Clockwise
Minute or Centisome (%) = 44.63
Right End: 2074658
Right Intergenic Region

Name: flu_yeeR

Length: 120 bp gap

Orientation: Codirectional+

Left_end: 2074659

Right_end: 2074778

Centisome: 44.70

The annotated start codon for flu has been confirmed by mutagenesis; an alternative upstream in-frame ATG start codon (52 codons before the annotated start and lacking an obvious RBS) functions in vivo to drive a lacZ protein fusion and thus should result in some Ag43 with a signal peptide twice as long as the that produced from the annotated start codon but since a cleavable 104 signal peptide is very unusual the annotation of this alternate start codon will await further studies for confirmation; the upstream isrC sRNA is produced by premature termination of the flu(agn43) mRNA 5' UTR and may regulate csrD expression (Wallecha, 2014). The Ag43 alpha and beta subunits are formed by cleavage at residue 551. Flu (Ag43) can be glycosylated in non-K-12 strains. Binds TrxA (Kumar, 2004).

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BamHI EcoRI HindIII