GenePage for the dgcN gene of Escherichia coli K-12
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Primary Gene Name: dgcN EcoGene Accession Number: EG12880 K-12 Gene Accession Number: ECK2601 MG1655 Gene Identifier: b2604 |
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Gene Name Mnemonic: Diguanylate cyclase Alternate Gene Symbols: yfiN Description: Membrane-anchored diguanylate cyclase |
| MW: 45989.48 ---------408 aa |
Pre-Run BlastP UniProt Pre-Run BlastP NR+Env |
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Left End: 2742383 Left Intergenic Region Name: yfiR_dgcN Length: 11 bp overlap Orientation: Codirectional+ Left_end: 2742383 Right_end: 2742393 Centisome: 59.08 |
Genomic Address Clockwise Minute or Centisome (%) = 59.08 |
Right End: 2743609 Right Intergenic Region Name: dgcN_yfiB Length: 15 bp gap Orientation: Codirectional+ Left_end: 2743610 Right_end: 2743624 Centisome: 59.11 |
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In the E. coli UPEC strain UTI89, YfiR is a redox-sensitive, DsbA-dependent, periplasmic regulator of the diguanylate cyclase DgcN(YfiN) (Hufnagel, 2014; Hufnagel, 2017). DgcN(YfiN) has an N-terminal predicted periplasmic domain flanked by two TM segments and has a C-terminal cytoplasmic diguanylate cyclase GGDEF domain. The N-terminal domain is probably a signal input domain because there is a HAMP linker signal transduction domain (residues 183-236) between the periplasmic domain and the GGDEF domain. The HAMP linker signal transduction domain is detected using EBI's InterProScan as matching ProSite PS50885, as annotated in UniProtKB P46139, but has an insignificant match to a HAMP linker Pfam profile (PF00672, E=0.15) using NCBI's CD-Search. The P. aeruginosa ortholog DgcN(YfiN) is an inner membrane-associated diguanylate cyclase whose activity is negatively regulated by the periplasmic protein YfiR; the P. aeruginosa OM lipoprotein YfiB counteracts YfiR inhibition of DgcN(YfiN); P. aeruginosa DgcN(YfiN) regulates exopolysaccharide production (Malone, 2010). YfiR may interact with the predicted N-terminal periplasmic domain of DgcN(YfiN).
