GenePage for the dgcN gene of Escherichia coli K-12

Primary Gene Name: dgcN
EcoGene Accession Number: EG12880
K-12 Gene Accession Number: ECK2601
MG1655 Gene Identifier: b2604
Gene Name Mnemonic: Diguanylate cyclase
Alternate Gene Symbols: yfiN
Description: Membrane-anchored diguanylate cyclase
  # bp Upstream # bp Downstream
MW: 45989.48 ---------408 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2742383
Left Intergenic Region

Name: yfiR_dgcN

Length: 11 bp overlap

Orientation: Codirectional+

Left_end: 2742383

Right_end: 2742393

Centisome: 59.08

Genomic Address
Clockwise
Minute or Centisome (%) = 59.08
Right End: 2743609
Right Intergenic Region

Name: dgcN_yfiB

Length: 15 bp gap

Orientation: Codirectional+

Left_end: 2743610

Right_end: 2743624

Centisome: 59.11

In the E. coli UPEC strain UTI89, YfiR is a redox-sensitive, DsbA-dependent, periplasmic regulator of the diguanylate cyclase DgcN(YfiN) (Hufnagel, 2014; Hufnagel, 2017). DgcN(YfiN) has an N-terminal predicted periplasmic domain flanked by two TM segments and has a C-terminal cytoplasmic diguanylate cyclase GGDEF domain. The N-terminal domain is probably a signal input domain because there is a HAMP linker signal transduction domain (residues 183-236) between the periplasmic domain and the GGDEF domain. The HAMP linker signal transduction domain is detected using EBI's InterProScan as matching ProSite PS50885, as annotated in UniProtKB P46139, but has an insignificant match to a HAMP linker Pfam profile (PF00672, E=0.15) using NCBI's CD-Search. The P. aeruginosa ortholog DgcN(YfiN) is an inner membrane-associated diguanylate cyclase whose activity is negatively regulated by the periplasmic protein YfiR; the P. aeruginosa OM lipoprotein YfiB counteracts YfiR inhibition of DgcN(YfiN); P. aeruginosa DgcN(YfiN) regulates exopolysaccharide production (Malone, 2010). YfiR may interact with the predicted N-terminal periplasmic domain of DgcN(YfiN).

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BamHI EcoRI HindIII