GenePage for the casE gene of Escherichia coli K-12

Primary Gene Name: casE
EcoGene Accession Number: EG13115
K-12 Gene Accession Number: ECK2751
MG1655 Gene Identifier: b2756
Gene Name Mnemonic: CRISPR-associated
Alternate Gene Symbols: cas6e; cse3; ygcH
Description: CRISPR RNA precursor cleavage enzyme; CRISPR RNA (crRNA) containing Cascade antiviral complex
  # bp Upstream # bp Downstream
MW: 22292.99 ---------199 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2879788
Left Intergenic Region

Name: ygbT_casE

Length: 15 bp gap

Orientation: Codirectional-

Left_end: 2879773

Right_end: 2879787

Centisome: 62.04

Genomic Address
Counterclockwise
Minute or Centisome (%) = 62.04
Right End: 2880387
Right Intergenic Region

Name: casE_casD

Length: 14 bp overlap

Orientation: Codirectional-

Left_end: 2880374

Right_end: 2880387

Centisome: 62.05

CasE(YgcH) can cleave or facilitate autocleavage of CRISPR RNA (crRNA) precursors to ~67 nt fragments (Brouns, 2008). The Cascade antiviral complex is comprised of CasABCDE and short CRISPR RNAs (crRNAs) and requires the predicted helicase YgcB(Cas3) for activity (Brouns, 2008).

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BamHI EcoRI HindIII