GenePage for the dinQ gene of Escherichia coli K-12

Primary Gene Name: dinQ
EcoGene Accession Number: EG14431
K-12 Gene Accession Number: ECK4422
MG1655 Gene Identifier: b4613
Gene Name Mnemonic: Damage inducible
Alternate Gene Symbols: None
Description: UV-inducible membrane toxin; DinQ-AgrB type I toxin-antitoxin system
  # bp Upstream # bp Downstream
MW: 3020.84 ---------27 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3647705
Left Intergenic Region

Name: gor_dinQ

Length: 53 bp gap

Orientation: Convergent

Left_end: 3647652

Right_end: 3647704

Centisome: 78.59

Genomic Address
Counterclockwise
Minute or Centisome (%) = 78.59
Right End: 3647788
Right Intergenic Region

Name: dinQ_agrA

Length: 274 bp gap

Orientation: Divergent

Left_end: 3647789

Right_end: 3648062

Centisome: 78.59

DinQ is a 27 amino acid UV-inducible toxin that depolarizes the cell membrane, decreases ATP concentration, and affects nucleoid structure after UV exposure; DinQ and the agrB sRNA antitoxin constitute a novel type I-like toxin-antitoxin module that affects the cell response to UV-induced DNA damage; three lines of evidence demonstrate that the agrA sRNA antitoxin can neutralize the DinQ toxin: (1) an agrA deletion mutant is UV-sensitive, grows slowly, and is recessive, while the dinQ agrAB deletion strain is not UV-sensitive and grows normally (agrA single mutants have no phenotype, indicating that the agrA sRNA is inactive), (2) a clone with both agrA and dinQ does not display the UV-sensitivity caused by introducing a dinQ clone alone into wildtype cells, and (3) a dinQ clone cannot be introduced into an agrB mutant strain (Weel-Sneve, 2013). Immunoblots of chromosomal SPA-tagged DinQ failed to show a band, indicating that dinQ was not expressed under the conditions tested (Hemm, 2008; Hemm, 2010). LexA regulon. DinQ has a single predicted transmembrane helix.

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BamHI EcoRI HindIII