GenePage for the fnrS gene of Escherichia coli K-12

Primary Gene Name: fnrS
EcoGene Accession Number: EG31185
K-12 Gene Accession Number: ECK4511
MG1655 Gene Identifier: b4699
Gene Name Mnemonic: Fumarate and nitrate reduction sRNA
Alternate Gene Symbols: rydA
Description: FNR-activated anaerobic sRNA; mediates negative FNR regulation; Hfq-dependent
  # bp Upstream # bp Downstream
MW: 39155.12 ---------122 nt Pre-Run BlastN
Left End: 1409129
Left Intergenic Region

Name: ydaN_fnrS

Length: 95 bp gap

Orientation: Codirectional+

Left_end: 1409034

Right_end: 1409128

Centisome: 30.36

Genomic Address
Clockwise
Minute or Centisome (%) = 30.36
Right End: 1409250
Right Intergenic Region

Name: fnrS_dbpA

Length: 260 bp gap

Orientation: Codirectional+

Left_end: 1409251

Right_end: 1409510

Centisome: 30.36

FNR activates fnrS transcription anaerobically and then FnrS negatively regulates cydDC, metE, sodA, sodB and other targets, which are translationally repressed indirectly by FNR through FnrS in this manner; FnrS also activates at least one target gene (yhaO) (Boysen, 2010; Durand, 2010). FnrS was previously predicted to be an sRNA based on conservation, but no transcripts are made aerobically so no expression was detected (Wassarman, 2001). Deep sequencing of sRNAs from Salmonella identified the FnrS ortholog STnc580 (Sittka, 2008). The 5' end of FnrS was determined by primer extension and the 3' end is the last U of a predicted rho-independent terminator; the predicted 122 nt size of FnrS was confirmed by Northern blot (Boysen, 2010).

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BamHI EcoRI HindIII